Sabouraud Chloramphenicol Agar
Recommended for the selective cultivation of yeasts and moulds from clinical and non-clinical samples.
Sabouraud Chloramphenicol Agar is cited as Medium C and recommended for cultivation of yeasts and moulds.
This medium was described originally by Sabouraud (7) for the cultivation of fungi, particularly useful for the fungi
associated with skin infections. The medium is often used with antibiotics such as Chloramphenicol (1) for the isolation
of pathogenic fungi from materials containing large numbers of fungi or bacteria.
Tryptone and peptone provide nitrogenous and carbonaceous compounds, long chain amino acids, and other essential
growth nutrients. Dextrose provides an energy source. Chloramphenicol inhibits a wide range of Gram-positive and
Gram-negative bacteria which makes the medium selective for fungi (5). The low pH favors fungal growth and
inhibits contaminating bacteria from clinical specimens (6).