How to work with a sampler (micropipette) in the laboratory ?

Summary

A sampler (micropipette) is one of the most important and essential laboratory equipment used to remove and transfer solutions and liquids in microliter quantities. In fact, the sampler is a simple pipette that can move liquid samples (with a small scale).

8/26/2023 8:59:20 AM
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How to work with a sampler (micropipette) in the laboratory ?

A sampler (micropipette) is one of the most important and essential laboratory equipment used to remove and transfer solutions and liquids in microliter quantities. In fact, the sampler is a simple pipette that can move liquid samples (with a small scale).

Application of the sampler

A sampler or micropipette or pipettor is one of the devices that are used to move and suck solutions in molecular biology laboratories with a range between 0.2 and 10,000 microliters.

 

What is a sampler?

How to work with sampler

Today, the basis and method of working with a laboratory sampler is done in two ways, mechanical and electronic. In fact, mechanical samplers with the help of hand power and electronic samplers with the help of electric power perform the act of emptying and suctioning the samples.

  • Also, to work with the sampler, it is necessary to connect a suitable sampler head to the device.
  • Then do the Pre-Wetting process.
  • After that, it is necessary to push the chassis of the sampler to part 1. And in this position (without leaving the chassis), move the sampler vertically into the desired liquid.
  • Then by releasing the chassis, slowly enter the solution into the laboratory sampler. (If you see air bubbles inside the sampler, it is necessary to repeat this process again.)
  • To empty the amount of liquid into the sampler, you must first stick the head of the sampler to the wall of the desired container.
  • Then press the sampler button until part 1. (After a few seconds to empty all the liquid, it is necessary to lower the chassis of the device to part 2.)
  • After emptying the liquid from the sampler, it is necessary to remove the micropipette device without touching the sampler head with the wall of the container.
  • Then release the chassis and remove the sampler head from the device and throw it away.
  • And put it in your base for another use.

Types of laboratory samplers

Nowadays, micropipettes or laboratory samplers are produced in various designs, such as electric and manual, based on the volume of solution in two types of variable and fixed samplers, and in terms of accuracy and application in two models of multi-channel or single-channel samplers and in laboratory centers. It is sold.

  • Variable sampler

The variable sampler is a suitable laboratory micropipette that is used to remove and move different volumes of liquid samples.

  • Fixed sampler

Fixed sampler are also other micropipette samples based on solution volume. This model of sampler is also used to take a known and specified volume of liquids.

It should be noted that although fixed samplers have higher accuracy than variable samplers, variable micropipettes are used more because they have a higher volume range.

  • Multi-channel sampler

This model of sampler is also suitable for moving liquids with high precision, having two models, 8-channel sampler and 12-channel sampler. In fact, this model of micropipette, like a normal sampler, is suitable for sampling solutions with a volume of several microliters to several hundred microliters.

  • Single channel sampler

This sampler is also one of the most important micropipettes used in research centers and medical laboratories, which is always used to distribute and move liquids with measured volumes.

 

Types of laboratory samplers

How to store laboratory sampler

  • It is better to use a special base to store samplers.
  • Depending on the amount of use, clean and lubricate the sampler regularly. (To clean all the external parts of the sampler, you can use a soap solution and then distilled water, and then dry it at room temperature. To clean the internal parts, follow the instructions written in the manual.)
  • If the sampler is full of liquid, do not place the device horizontally, because in this case, the sampler will not work properly when the solution enters the shaft of the device.
  • When working with the sampler, make sure that the micropipette head is tight so that the device does not draw air.
  • Avoid hitting the micropipette while working with the device, as this will throw the sampler out of calibration.

How to work with a sampler (micropipette) in the laboratory ?