Cell culture is a process in which cells are grown. This growth is mostly done in a controlled and favorable environment. In fact, to understand this issue more easily, it can be said that cell culture is the process of growing eukaryotic cells in a sterilized environment. It should also be noted that each cell has its own culture environment and must be cultured in its own environment. For examp
Cell culture is a process in which cells are grown. This growth is mostly done in a controlled and favorable environment. In fact, to understand this issue more easily, it can be said that cell culture is the process of growing eukaryotic cells in a sterilized environment. It should also be noted that each cell has its own culture environment and must be cultured in its own environment. For example, for cultivation, artificial environments must consist of substances such as amino acids, hormones, sugars, gases, minerals, etc. Animal cells are usually cultured at a temperature of 37 degrees and in the necessary equipment.
The environment for cell culture is very important. For this reason, it is very important that the cultivation environment has appropriate conditions. For example, the culture medium should not be placed in high traffic places or laboratories such as microbiology. The laboratory must be made of equipment that can be disinfected. For example, stainless steel is suitable for cabinets and tables. The culture environment should always be sterile and disinfected. It is better to use an air conditioner in the laboratory environment. Liquid culture should be stored in an environment with a temperature of 4 degrees Celsius. Therefore, it is necessary to have a refrigerator and freezer for this environment.
Cell culture has different types, including organ culture, all tissue parts culture, and cell culture.
In cell culture, the culture loses its original structure and changes occur in it. And it can be said that they are out of their original context.
The meaning of tissue culture is that part of the tissue is in contact with the liquid surface. The purpose of cell migration in this culture is growth.
In the organ tissue, the tissue is in contact with the surface of the gas liquid and finally, unlike cell culture, its three-dimensional appearance is preserved.
Cells grow and multiply to a certain extent. But from somewhere later, these cells are exposed to the so-called aging process. One of the disadvantages of the aging process is that cell division no longer occurs. To prevent this, the cells can be placed in a cell incubator . This environment should have a suitable temperature and a suitable gas mixture. Of course, it should be noted that the cultivation conditions of each cell are different from another cell. There are many parameters to show the differences in the culture medium of cells, which can be mentioned as pH, glucose concentration...
In this medium, by adding FBS to the basic medium, we are able to grow most of the cells. This environment is called Eagles Minimum Essential Medium . This environment was recorded for the first time by a scientist named (Eagles) .
In this environment, by mixing the culture medium with sugar and salt, we are able to optimize the culture medium. This environment is derived from the EMEM environment .
